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. 2002 Mar 18;156(6):1089–1098. doi: 10.1083/jcb.200111107

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — Cytochrome c localization in BG2 cells is not altered after serum deprivation. (A) Untreated BG2 cells or those deprived of serum and insulin for 24 h were fixed and costained with anti-DRONC polyclonal antibody and anti–cytochrome c monoclonal antibody. After incubation with FITC- (DRONC) or rhodamine- (cytochrome c) coupled secondary antibodies, cells were visualized by fluorescence microscopy using appropriate filters. The last column depicts merged images of the DRONC and cytochrome c costaining. (B) BG2 cells deprived of serum and insulin for various lengths of time were fixed and immunostained with anti-DRICE polyclonal antibody. As the anti-DRICE antibody only detects the active processed form of DRICE, immunofluorescence is seen in apoptotic cells only.