Figure 3.

Different domains mediate association of Cdc20–GFP to different subcellular locations. The region from 110–167 amino acids appears to contain a domain required for accumulation of the fusion protein at interphase and mitotic centrosomes (arrowheads). The complete WD-40 array appears to be required for localization of the fusion protein to kinetochores. CTRS, centrosomes; KIN, kinetochores; SPD, spindle fibers. Bar, 5 μm.