Figure 6.
Reduced MyoD protein levels in GFP+mut 3′-UTR cells do not result from differential MyoD transcription or RNA stability. MyoD expression in control C2C12 cells, the GFP+wt 3′-UTR pool, and the GFP+mut 3′-UTR pool after 0, 6, and 12 h in differentiation media were analyzed on Northern blots and RNA slot blots. A Northern blot is shown, and the graph shows the behavior of MyoD steady-state RNA levels (normalized to gapdh levels) after 6 and 12 h in differentiation media relative to levels present in proliferating cells. These results were quantified from three slot blot experiments.