Figure 2.
Effect of brefeldin A on clathrin/AP-2 recruitment. Clathrin/AP-2 recruitment was performed as described in Fig. 1 in the presence or absence of ATP/GTPγS and brefeldin A (BFA). Samples were analyzed by quantitative Western blot analysis using antisera against α-adaptin, AP180, synaptotagmin I as a membrane marker, and Hsc70 as a control.
