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. 2003 Jul 7;162(1):113–124. doi: 10.1083/jcb.200301006

Figure 2.

Figure 2.

Effect of brefeldin A on clathrin/AP-2 recruitment. Clathrin/AP-2 recruitment was performed as described in Fig. 1 in the presence or absence of ATP/GTPγS and brefeldin A (BFA). Samples were analyzed by quantitative Western blot analysis using antisera against α-adaptin, AP180, synaptotagmin I as a membrane marker, and Hsc70 as a control.