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. 2004 Dec 20;167(6):1147–1159. doi: 10.1083/jcb.200402033

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — MURF1 inhibits adrenergic agonist-induced PKCε activity (but not PKCβII activity) in the particulate fraction of NRVM. (A–C) After infection with Ad.GFP or Ad.MURF1 for 24 h in serum-free medium, NRVM were induced with PE or PMA for 15 min and subjected to subcellular fractionation. The detergent-soluble (S) and particulate (P) fractions were immunoprecipitated with anti-PKCβII (A) or anti-PKCε antibody (B and C) and were subjected to in vitro kinase assays using histone H1 and γ[³²P]ATP. (D and E) The results from densitometric scanning of kinase assays from three independent experiments are presented as means ± SEM of PKCε (D) or PKCβII (E) activity in the particulate fraction compared with soluble fraction. *, P < 0.05 compared with control.