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. 2004 Dec 20;167(6):1147–1159. doi: 10.1083/jcb.200402033

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Copyright © 2004, The Rockefeller University Press

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Figure 3. — MURF1 does not alter PE- and PMA-induced translocation of PKCβII from cytosol to the perinuclear region in NRVM. NRVM were infected with Ad.GFP (A–C) or Ad.MURF1 (D–F) for 24 h in serum-free medium followed by induction with PE or PMA for 15 min. The cells were fixed and incubated with anti-RACK1 (blue) and anti-PKCβII (red) for 2 h. (G) Quantitative determination of perinuclear PKCβII staining under the indicated conditions.