Table III. Inhibition of early colony formation by pre-treatment with anti-integrin subunit antibodies.
| Treatment | Colonies/lung ± SD | Range (# of colonies) |
# of mice | t test against no antibody |
|---|---|---|---|---|
| No antibody | 2.25 ± 1.71 | 0–5 | 12 | - |
| Anti-β1 blocking | 0 | 0 | 7 | 0.001 |
| Anti-β1 nonblocking | 2.43 ± 1.72 | 1–6 | 7 | - |
| Anti-α3 (P1B5) | 0.71 ± 0.76 | 0–2 | 7 | 0.016 |
| Anti-α3 (ASC-1) | 0.71 ± 0.95 | 0–2 | 7 | 0.022 |
| Anti-α6 | 1.86 ± 2.27 | 0–5 | 7 | - |
HT1080-GFP cells were treated with the indicated antibodies for 0.5 h before injection as described in the Materials and methods section. After washing, 5 × 105 cells were injected into the tail vein of nu/nu mice. The numbers of colonies were counted after scanning the entire surface of the left lobe of the isolated mouse lung. A two-tailed t test is shown only for those with a significant difference as compared to the no antibody control.