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. 2004 Feb 2;164(3):451–459. doi: 10.1083/jcb.200311112

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Expression of α2-3–linked and α2-6–linked sialic acid in T-syn ⁺ ^/ ⁺ and T-syn ⁻ ^/ ⁻ embryos. (A–H) Sections of the indicated tissues were pretreated with or without sialidase and stained with Maackia amurensis hemagglutinin (MAH), which recognizes α2-3–linked sialic acid, or with Sambucus nigra (SNA), which recognizes α2-6–linked sialic acid. Note that MAH binds much less to T-syn ^−/− embryos than to T-syn ^+/+ embryos. Pretreatment of the sections with sialidase eliminated binding of MAH and SNA, confirming their specificity. Bars, 50 μm.