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. 2004 Sep 13;166(6):877–887. doi: 10.1083/jcb.200405004

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Optimum substrate modulus for myotube differentiation. Myoblasts that have fused to myotubes on collagen-coated PA substrates exhibit a striation tendency that is strongly dependent on the substrate's elastic modulus. The percentage of total cells (n > 15 for each sample) exhibiting actin-myosin striation on the collagen-coated PA gels showed an optimum gel modulus of E* = 12 kPa based on simple Gaussian fits with common means and SDs (∼2.5–3 kPa) for 2 and 4 wk. Circles represent differentiation of cells grown on IPN-patterned glass. Error bars are ± SEM.