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. 2005 Jun 6;169(5):745–753. doi: 10.1083/jcb.200411129

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Transfected hADAR2, but not transfected hADAR1 or ADAR1C-Term, is functional in the nucleolus. Histograms show the percentage of RNA editing at each edited adenosine after transient transfection in NIH-3T3 cells with the indicated constructs (A–C). Values represent the mean of three independent experiments (n = 3; error bars represent SD). Note: because the Q/R site of GluR-B is specifically edited by ADAR2, the editing efficiency of Pol I and Pol II/GluR-B (Q/R) upon ADAR1 transfection was not determined (ND). (A, right) Primer extension analysis of RNA editing at the C- and D-sites. The nucleotide sequence of the 5′ end-labeled (³²P) oligonucleotide sense primer, the antisense strand of the RT-PCR template, and the positions of the editing sites are indicated. P, primer. (D) Subcellular localization of GFP-ADAR2 and GFP-ADAR1C-Term in transiently transfected NIH-3T3. Nucleoli are visualized by a fluorescent oligonucleotide probe (Cy5) directed against U3 snoRNA.