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. Author manuscript; available in PMC: 2007 Dec 30.
Published in final edited form as: J Neuroimmune Pharmacol. 2007 Jun 27;2(3):251–258. doi: 10.1007/s11481-007-9081-3

Fig. 2.

Fig. 2

CXCR4 expression and CXCL12 signaling during NPC differentiation. NPC, neuronal differentiated (N-diff), and astrocyte differentiated (A-diff) cells were harvested and stained with anti-CXCR4-PE. The number of CXCR4 positive cells was determined by flow cytometry. Representative FACS data for undifferentiated NPC (a) N-diff (b) and A-diff (c) showing the number of CXCR4 positive cells. d The average percentage of CXCR4+ cells of four donors±SEM. CXCL12 production during neuronal and astrocyte differentiation as determined by ELISA (n=3) (e). A cAMP accumulation assay (f) was preformed to show the responsiveness of NPC, N-diff, and A-diff cultures to CXCL 12-mediated inhibition of Forskolin-stimulated cAMP accumulation. Representative data for three separate experiments with NPC, N-diff, and A-diff from three separate donors (*p<0.05 compared to N-diff; **p<0.05 compared to NPC)