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. 2007 Sep 4;27(21):7511–7521. doi: 10.1128/MCB.00753-07

FIG. 3.

FIG. 3.

Different acidic residue concentrations account for the differential binding affinities of the ETGE and DLG motifs to Keap1. Representative ITC titration profiles of the titration of Keap1-DC with Neh2(ΔETGE,D27A) (A), Neh2(ΔETGE,D29A) (B), Neh2(Δ1-33,D77A/E79A) (C), Neh2(ΔETGE,Q26A) (D), Neh2(Δ1-33,D77A) (E), and Neh2(Δ1-33,E79A) (F). The top graphs represent the raw ITC thermograms, and the bottom graphs represent the fitted binding isotherms. The integrated binding isotherms are plotted against the molar ratio of different mutants of Neh2 to Keap1-DC.