FIG. 7.
Autolysis profiles upon walKR induction. Strain ST1000 was grown in TSB without IPTG (⧫) or with 0.05 mM IPTG (▪), or 1 mM IPTG (•) to an OD600 of 1. The cells were then pelleted and resuspended in PBS to test the effects of autolysis-inducing agents. Triton- and lysostaphin-mediated lysis of staphylococci was measured as the decline of OD600 over time. (A) Autolysis determined at 30°C in the presence of 0.1% Triton X-100. (B) Autolysis determined at 37°C in the presence of 200 ng/ml lysostaphin.