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Effect of walKR induction on transcription of genes involved in cell wall degradation. Total RNA was extracted from ST1000 cells grown in TSB supplemented with 0.05 mM or 1 mM IPTG. After reverse transcription, specific cDNAs were quantified by qRT-PCR. The results are expressed as the means and standard deviations of quadruplicate experiments using primers specific for target genes and 16S rRNA (normalizing gene).
