FIG. 2.
Tuning the membrane recognition signal of the artificial one-component P450. (a) Construction of the reengineered one-component P450 enzymes (E2 to E5 enzymes) based on the E1 enzyme. (b) In vivo redox activity of E. coli expressing the fine-tuned artificial E2, E3, E4, and E5 P450 enzymes upon the addition of naringenin substrate to accumulate genistein in TB medium. (c) Synthetic sequences, ɛ and ϕ, used to replace the deleted N-terminal domain of the native IFS1. Nucleotide or amino acid substitutions in parental sequences (17A1c and LacZα) are indicated by boldface letters. (d) Growth profiles of the E. coli TOP10F′ strain expressing C, E1, E2, E3, E4, and E5 during the bioconversion of naringenin substrate in TB medium.