Figure 11.
Loss of actin stress fibers and focal contacts in GE11-β1A cells expressing N19-RhoA. Double-immunofluorescence staining of GE11-β1A cells, 3–4 d after N19RhoA retroviral transduction. Cells were fixed and double-stained for either myc-tagged N19RhoA (upper panels) or vinculin (lower panels) with F-actin. Note that the cluster of four cells in the upper panels that express N19RhoA at high levels does not exhibit actin stress fibers, in contrast to the cells expressing no or little N19RhoA. The cluster of seven cells shown in the lower panels displays no vinculin-positive focal contacts. Basal and medial focal planes are as indicated. Bar, 20 μm.