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. 1999 Dec 13;147(6):1275–1286. doi: 10.1083/jcb.147.6.1275

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Overexpression of mACF7 COOH-terminal domain stabilizes cellular MTs. pFLAG-mACF7-C (A–D) and pFLAG-ABD (E and F) and transfected COS-7 cells were treated with nocodazole (10 μM) for 1.5 h before being fixed and double-labeled with monoclonal anti-FLAG M2 antibody (A, C, and E) polyclonal anti–Tyr-tubulin antibody (B), polyclonal anti–Glu-tubulin antibody (D), and polyclonal antitubulin antibody (F). The overexpressed mACF7-C protein stabilized MTs from depolymerization by nocodazole (compare positive FLAG-staining cell with its neighboring cells in A and B). Bar, 20 μm.