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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Virology. 2007 Apr 10;364(2):281–290. doi: 10.1016/j.virol.2007.02.022

Figure 2. The influence of CCR5 expression on the sensitivity to CCR5 inhibitors in PBMC from different donors.

Figure 2

PBMCs from six human donors were infected with the primary HIV-1 isolates SB106, SB119 and AK103. The relative IC50 values for inhibition by PRO 140 and SCH-D were determined (see text). (A) The gating employed in the flow cytometry analyses is depicted. The population of lymphocytes was gated in the side- versus forward-scatter plot. The CD4+ subpopulation of lymphocytes (blue in histograms) was then gated. (The red tracing in the histograms represents the isotype control.) The median CCR5 fluorescence intensities of these CD4+ lymphocytes were used for the correlation. (B) The correlation between mean relative p24 production and CCR5 expression was weak, R2 = 0.56. (B) The correlation of PRO 140 IC50 versus CCR5 expression was strong, R2 = 0.99. (C) The correlation of SCH-D IC50 versus CCR5 expression was moderate, R2 = 0.64.