Figure 2.
Dynamics of β-catenin and stabilized β-catenin (β-cat*) at centrosomes. (A) FRAP of GFP-β-catenin (gray montage) at centrosomes marked by RFP-pericentrin. Arrow marks photobleach region. Bar, 5 μm. (B) Fluorescence recovery profile of data in A. (C) Representative fluorescence recovery profile of GFP-β-catenin mutated in four GSK3β phosphorylation sites (β-cat*) at centrosomes. (D) Summary of average fluorescence recovery and t1/2 of GFP-β-catenin (blue; n = 4) and GFP-β-cat* (orange; n = 6) at centrosomes. Diffusion alone is shown as a black triangle (n = 2). Average of experiments is shown ± standard deviation. Student’s t-test was performed for recovery of β-cat and β-cat* with P = 0.011.