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. 2007 Dec 14;28(5):798–809. doi: 10.1016/j.molcel.2007.09.020

Figure 4.

Figure 4

Persistent Monoubiquitinated FANCD2 and Not PCNA Is Responsible for DNA Damage Sensitivity

(A) FANCD2 (top) and PCNA (bottom) western blot of WT, ΔUSP1, hPCNA-K164R knockin, and hPCNA-K164R ΔUSP1 cell extracts.

(B) Colony survival assay with the same strains exposed to low doses of cisplatin or UV light. Error bars represent one SD.

(C) FANCD2 (top) and PCNA (bottom) western blot of WT, ΔUSP1, ΔFANCL, and ΔFANCLΔUSP1 cell extracts.

(D) Colony survival assay with the same strains exposed to low doses of cisplatin (top) and high doses of UV light (bottom). Error bars represent one SD.

(E) Table showing doubling times and the prevalence of apoptotic cells in the strains tested in this section.