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. 1999 Mar 8;144(5):903–914. doi: 10.1083/jcb.144.5.903

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Protein levels of Bak measured by Western blotting using the monoclonal antibody Ab-1 to Bak in CEM-C7A (A, top) or Jurkat cells (B, top) after treatment with STS (250 nM) for the times indicated. Blots for Bcl-2 and actin are also shown. The results are representative of three independent experiments. The middle panels show FCM-generated frequency histograms of Bak Ab-1-associated fluorescence in control, untreated cells (filled histogram) and after STS treatment for 4 h (open histogram). The histogram for the irrelevant antibody control (gray line) is directly superimposable upon the control, untreated histogram. The bottom panels show equivalent data for Bcl-2-associated fluorescence; the histogram for the irrelevant antibody is in the first decade (not shown). Results shown are representative of at least three repeat experiments.