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. Author manuscript; available in PMC: 2007 Dec 17.
Published in final edited form as: FEBS Lett. 2007 Oct 23;581(27):5315–5320. doi: 10.1016/j.febslet.2007.10.020

Figure 3. PM-induced upregulation of CDK inhibitor, p21 mediates PM-induced G1 arrest in AEC.

Figure 3

Figure 3

Figure 3

3a: PM caused progressive increase in p21 levels after 4h. The levels of p27 were modestly increased after 4h. 3b: p21 activity was increased by 2-fold as assessed by ELISA in AEC exposed to PM. 3c, 3d: siRNAp21 prevented PM-induced downregulation of cyclin A and phosphorylated-cyclin E; blocked the upregulation of p21; and prevented PM-induced AEC arrest. *p<0.05 control vs. PM (Scramble); †p<0.05 PM (Scramble) vs. PM (SiRNAp21) G1 Phase; n=3. 3e: Western blot with immunoprecipitation of cyclin E shows increase in p21 on exposure to PM, while siRNA to p21 caused reduction in cyclin E.