Figure 2.
Isoform specificity of syntrophin antibodies. Antibodies were characterized by immunoblotting samples enriched in individual syntrophin isoforms (as indicated above lanes). α1-, β1-, and β2-syntrophins were solubilized from crude membrane preparations from skeletal muscle, liver, and kidney, respectively, in 1% SDS to disrupt potential multi-syntrophin complexes. After addition of excess Triton, syntrophins were immunoaffinity purified with the appropriate antibody (Abs SYN17, SYN37, and SYN28). Blots were probed with mAb SYN1351 or biotinylated polyclonal antibodies as indicated. Labeling with each polyclonal antibody was eliminated by preincubation with the appropriate antigenic peptide (not shown). Positions of two molecular mass markers (52 and 59 kD) are shown on the right in the top panel.