Abstract
Peritoneal exudate cells from animals exhibiting delayed hypersensitivity are inhibited from migrating in vitro by specific antigen. This inhibition is completely abolished by pretreatment of the sensitive cells with trypsin. The action of trypsin is prevented by soybean trypsin inhibitor. The results of experiments with mixtures of normal and sensitive cells suggest that trypsin alters an immunologic capacity of the sensitive cells. Trypsinized sensitive cells are capable of passively transferring delayed hypersensitivity and peritoneal cells taken from recipient animals are inhibited from migrating in vitro by specific antigen. These results suggest that the cells rapidly resynthesize the material removed by trypsin. The possible nature of the material removed by trypsin is discussed.
Full Text
The Full Text of this article is available as a PDF (692.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUER J. A., Jr STONE SH: Isologous and homologous lymphoid transplants. I. The transfer of tuberculin hypersensitivity in inbred pigs. J Immunol. 1961 Feb;86:177–189. [PubMed] [Google Scholar]
- BLOOM B. R., HAMILTON L. D., CHASE M. W. EFFECTS OF MITOMYCIN C ON THE CELLULAR TRANSFER OF DELAYED-TYPE HYPERSENSITIVITY IN THE GUINEA PIG. Nature. 1964 Feb 15;201:689–691. doi: 10.1038/201689a0. [DOI] [PubMed] [Google Scholar]
- BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
- DAVID J. R., AL-ASKARI S., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS. J Immunol. 1964 Aug;93:264–273. [PubMed] [Google Scholar]
- DAVID J. R., LAWRENCE H. S., THOMAL L. DELAYED HYPERSENSITIVITY IN VITRO. II. EFFECT OF SENSITIVE CELLS ON NORMAL CELLS IN THE PRESENCE OF ANTIGEN. J Immunol. 1964 Aug;93:274–278. [PubMed] [Google Scholar]
- DAVID J. R., LAWRENCE H. S., THOMAS L. DELAYED HYPERSENSITIVITY IN VITRO. 3. THE SPECIFICITY OF HAPTEN-PROTEIN CONJUGATES IN THE INHIBITION OF CELL MIGRATION. J Immunol. 1964 Aug;93:279–282. [PubMed] [Google Scholar]
- GEORGE M., VAUGHAN J. H. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. doi: 10.3181/00379727-111-27841. [DOI] [PubMed] [Google Scholar]
- MOSCONA A. Rotation-mediated histogenetic aggregation of dissociated cells. A quantifiable approach to cell interactions in vitro. Exp Cell Res. 1961 Jan;22:455–475. doi: 10.1016/0014-4827(61)90122-7. [DOI] [PubMed] [Google Scholar]