Abstract
An in vitro technique is described for assessing the chemotactic activity of soluble substances on motile cells. Antibody-antigen mixtures when incubated (37°C) in medium containing fresh (i.e. non-inactivated) normal rabbit serum exert a strong chemotactic effect on rabbit polymorphonuclear leucocytes. Results are described which indicate that, when antibody-antigen complexes are incubated (37°C) in fresh serum, a heat-stable (56°C) substance (or substances) is produced which acts directly as a chemotactic stimulus on the polymorphs. This heat-stable chemotactic substance is not produced when antibody-antigen complexes are incubated in serum which has been heated at 56°C for 30 minutes.
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Selected References
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- HARRIS H. Role of chemotaxis in inflammation. Physiol Rev. 1954 Jul;34(3):529–562. doi: 10.1152/physrev.1954.34.3.529. [DOI] [PubMed] [Google Scholar]
- HIRSCH J. G., CHURCH A. B. Studies of phagocytosis of group A streptococci by polymorphonuclear leucocytes in vitro. J Exp Med. 1960 Mar 1;111:309–322. doi: 10.1084/jem.111.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PULVERTAFT R. J. Cellular associations in normal and abnormal lymphocytes. Proc R Soc Med. 1959 May;52(5):315–322. doi: 10.1177/003591575905200501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHELTON E., RICH M. E. Studies on mouse lymphomas. II. Behavior of three lymphomas in diffusion chambers in relation to their invasive capacity in the host. J Natl Cancer Inst. 1958 Jul;21(1):137–161. [PubMed] [Google Scholar]