Figure 7.
Type IV collagen distribution in a temperature-sensitive let-2 glycine substitution mutant. Staining of the temperature-sensitive glycine substitution mutant let-2(b246). Embryos grown at 25°C (A), 20°C (B), or 15°C (C) were stained with LET-2 antiserum. Staining with EMB-9 antiserum gives an identical pattern. At 25°C, type IV collagen is seen as intense intracellular spots in body wall muscle cells. Very faint staining of basement membranes is seen in some embryos. At 15 and 20°C, type IV collagen is distributed in an essentially wild-type pattern.