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. 1999 May 17;145(4):659–672. doi: 10.1083/jcb.145.4.659

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Pma1p defects caused by lst1Δ. (A) lst1Δ cells (CKY534) were photographed using differential interference contrast microscopy after growth at 37°C on YPD, pH 3.8. A montage of multibudded cells is shown. Cells of this type comprise ∼10% of a lst1Δ culture, but are never seen in wild-type grown under the same conditions. Bar, 10 μm. (B) Reduced capacity for proton pumping by lst1Δ cells. Wild-type (CKY443) and lst1Δ (CKY536) were grown to exponential phase in YPD medium, pH 6.8, at 37°C. Cells were incubated in water overnight and then suspended in 10 mM glycine buffer at pH 4.0. Proton efflux from the cells after addition of glucose was recorded as a decrease in the pH of the external medium. Based on the average rate of change in pH over the first 5 min after glucose addition, lst1Δ cells exhibited 65% the rate of proton efflux as wild-type.

Pma1p defects caused by lst1Δ. (A) lst1Δ cells (CKY534) were photographed using differential interference contrast microscopy after growth at 37°C on YPD, pH 3.8. A montage of multibudded cells is shown. Cells of this type comprise ∼10% of a lst1Δ culture, but are never seen in wild-type grown under the same conditions. Bar, 10 μm. (B) Reduced capacity for proton pumping by lst1Δ cells. Wild-type (CKY443) and lst1Δ (CKY536) were grown to exponential phase in YPD medium, pH 6.8, at 37°C. Cells were incubated in water overnight and then suspended in 10 mM glycine buffer at pH 4.0. Proton efflux from the cells after addition of glucose was recorded as a decrease in the pH of the external medium. Based on the average rate of change in pH over the first 5 min after glucose addition, lst1Δ cells exhibited 65% the rate of proton efflux as wild-type.