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. 1998 May 18;141(4):929–942. doi: 10.1083/jcb.141.4.929

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PLAP and hTfR patching depends on membrane cholesterol. BHK cells transiently expressing PLAP (A) and hTfR (B) were depleted of membrane cholesterol using cyclodextrin extraction. PLAP and hTfR were cross-linked using anti-PLAP and anti-hTfR mAb, respectively, followed by rhodamine-coupled secondary antibodies. Patching of PLAP as well as hTfR is inhibited under these conditions. Bar, 5 μm.