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. 2007 Feb 19;204(2):441–452. doi: 10.1084/jem.20061737

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Copyright © 2007, The Rockefeller University Press

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Figure 4. — Impaired production of IL-12, -6, and TNF-α in dyslipidemic mice is restricted to the CD8α⁻ myeloid DC subset. Splenic DCs were isolated from HFCD-fed C57BL/6 (B6) or apoE ^−/− mice and stimulated with CpG for 6 h. (A) Representative dot plots from cells stimulated with 100 nM CpG are shown. (B) The proportion of IL-12p40–producing cells in CD8α⁻ and CD8α⁺ DC subsets after stimulation with the indicated doses of CpG. (C) Splenic CD8α⁻ DCs and (D) CD8α⁺ DCs were sorted by flow cytometry from C57BL/6 and apoE ^−/− mice were fed either a chow or HFCD for 10 wk and stimulated with CpG and anti-CD40. Supernatants were collected after 20 h of culture and assayed for IL-12p40, -12p35, -6, and TNF-α by ELISA. Error bars represent the mean ± the SD.