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. 2007 Oct 29;204(11):2667–2677. doi: 10.1084/jem.20062376

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Copyright © 2007, The Rockefeller University Press

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Figure 7. — Endogenous CD8 T cells exhibit regulation of CD8 expression, tetramer binding, and antigen sensitivity. B6 mice were infected with VV-OVAp and spleens were analyzed 5 and 7 d after infection. Peptide-specific CD8 T cells (defined by K^b/OVA tetramer staining) were analyzed for the level of tetramer binding (A) and CD8 expression level (B). (C) The CD8 dependence of tetramer binding at both time points was assessed by addition of blocking anti-CD8α antibody. Each column or dot represents an average of three or four mice, and error bars indicate the SD. (D) Antigen sensitivity of K^b/OVA-specific endogenous CD8 T cells was assessed by stimulation for IFN-γ production, as in Fig. 4. The percentage of IFN-γ producing CD8 T cells as a function of OVAp concentration is indicated on the left side of the graph. On the right side, the percentage of K^b/OVA tetramer-positive cells is indicated, to reflect the frequency of antigen-specific cells in the populations. Data show the average (plus the SD) of three mice per group, and similar findings were observed in an independent experiment.