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. Author manuscript; available in PMC: 2007 Dec 5.
Published in final edited form as: Gene Expr Patterns. 2007 Mar 23;7(6):680–699. doi: 10.1016/j.modgep.2007.03.002

Figure 4. Comparison of low resolution annotation of TS23 kidney whole mounts with the structures discernable in neonatal kidney sections.

Figure 4

Analysis of whole mount in situ hybridisation (WISH) of kidney and ureter at TS23 reveal 8 readily annotated patterns corresponding to the ureteric tip (A, Wnt11), ureteric trunk (B, Wnt7b), cap mesenchyme (C, Cited1), early tubule (D, Wnt4), late tubule (E, Slc12a3), renal vasculature (F, Sox18), renal interstitium (G, Foxd1) and ureter (H, Foxa1). Section in situ hybridisation of neonatal kidneys (I-P) for the same genes confirms the accuracy of these earlier annotations. Expression in deeper or more mature structures within the kidney revealed by SISH is not obvious from TS23 whole mount images either due to lack of expression of mature structures at an earlier stage or masking of deeper signal by strong superficial signals. Scale bar = 40μM. Many of the terms used to annotate whole mount material at TS23 are group terms. Images courtesy of Jing Yu.