Figure 2.
Phenotype of Mttp−/− embryos. (A) Mttp+/+ embryo at E9.5. (B) An E9.5 Mttp−/− embryo with incomplete anterior closure of the neural tube. (C) An E10.5 Mttp−/− embryo that appeared necrotic, and had incomplete anterior closure of the neural tube. (D) An E14.5 Mttp−/− embryo with exencephalus. (E and F) Hematoxylin and eosin staining of yolk sac membranes from Mttp+/+ (E) and Mttp−/− (F) embryos at E10.5. There were reduced numbers of blood cells in the blood islands of Mttp−/− membranes compared with the Mttp+/+ membranes. Note the vacuolization at the basolateral surfaces of the Mttp−/− visceral endoderm (arrow).