FIG. 5.
Replication dynamics in the presence of HU. (A and C) Replication profiles for chromosomes VI and XI, respectively. The synchronous culture was treated with HU as described for Fig. 4 and in the text. Chromosome profiles and significant origin peaks were identified as described for Fig. 3 and in the text. The bottom panels show the accumulation of HL DNA at 40 (purple), 80 (red), 120 (blue), 240 (gold), and 300 (green) minutes postrelease plotted against the chromosomal coordinate. The yellow circle identifies the centromere. The top panels indicate the sample time(s) at which replication origins (peaks in the chromosome profile) are active. (B and D) Control experiment as described for Fig. 3. (E) Cumulative origin count during S phase is plotted as a function of the percentage of the genome that is hybrid in density. See the Fig. 3 legend for details.