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. 2007 Dec 3;179(5):869–879. doi: 10.1083/jcb.200707120

Figure 2.

Figure 2.

MCAK suppresses tension in a dose-dependent manner. (A) Addition of exogenous MCAK (GCPBM) to centromeres decreases centromere separation significantly from controls (GCPB and GCPBMhyp) in living cells (P < 0.0001). Expression of mRFP motorless MCAK (RMLM), RAMFLhyp, or depletion of MCAK with siRNA significantly increases sister centromere separation (as visualized by GCPB; P < 0.0001). Between five and eight live sister centromeres per cell were measured for 5–15 successive frames (20 s per frame). Each bar (SEM [error bars] is shown) represents three to six different cells from at least two different experiments on different days. (B) Sister centromeres in metaphase cells expressing GCPBM appear quite close together. See Video 1 (available at http://www.jcb.org/cgi/content/full/jcb.200707120/DC1). (C) Sister centromeres in metaphase cells coexpressing GCPB and RAMFLhyp appear to be under significantly more tension (P < 0.0001). See Video 2. (D) Histograms of sister centromere separation for GCPB, GCPBM, GCPBMhyp, and RAMFLhyp. Overlaid comparisons are shown between GCPBM and GCPBMhyp, the two controls (which are not significantly different; P = 0.40), and GCPB- and GCPB/RAMFLhyp-coexpressing cells.