Table 3.
Infectivity of RA-associated B19
| Source | Ab | % VP-1-positive cells
|
|||||
|---|---|---|---|---|---|---|---|
| BMC | Tonsil | Fib | U937 | THP-1 | UT-7 | ||
| SVC from RA1 | — | 0.4 | 1.0 | 0.4 | |||
| SVC from RA1 | 1F5 | 0.4 | 1.1 | 0.4 | |||
| SVC from RA1 | PAR3 | <0.01 | 0.05 | <0.01 | |||
| SVC from RA4 | — | 1.6 | 2.1 | ||||
| SVC from RA4 | 1F5 | 1.5 | |||||
| SVC from RA4 | PAR3 | <0.01 | |||||
| SVC from OA1 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | ||
| SVC from OA2 | <0.01 | <0.01 | <0.01 | <0.01 | |||
| BMC from RA5 | 2.3 | <0.01 | 2.7 | ||||
| BMC from RA6 | — | 0.6 | |||||
| BMC from RA6 | 1F5 | 0.6 | <0.01 | ||||
| BMC from RA6 | PAR3 | <0.01 | |||||
| BMC from AML1 | <0.01 | <0.01 | |||||
B19-negative BMC, tonsil cells (tonsil), synovial fibroblasts (Fib), U937 cells, THP-1 cells, or B19-permissive cell line UT-7 cells were cocultured with SVC or BMC in double-chamber culture system, and the expression of VP-1 was evaluated by an inflammation study 3 days after. VP-1-positive cells are seen among these cells except synovial fibroblasts. The addition of neutralizing antibody (Ab) for B19 at the initiation of the culture causes marked decrease of VP-1-positive cells in the culture.