Figure 3.
Traces of uncapped mRNA species with short poly(A) tails can also be detected for three other mRNAs. PCK, ALB, and TAT mRNAs were analyzed as described for SAA mRNA in Fig. 2, except that (i) the RNA preparation originated from unstimulated rat hepatoma cells, (ii) the amplifications were performed with primers 1 (i.e., the reverse transcription primer) and 3, and (iii) the number of amplification cycles performed to analyze ALB mRNA was 25 and 29, respectively, for the capped and uncapped species.