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. Author manuscript; available in PMC: 2007 Nov 15.
Published in final edited form as: Dev Dyn. 2007 Sep;236(9):2475–2484. doi: 10.1002/dvdy.21244

TABLE 1.

Mean Atrial Area of Stage 46 Embryos Injected With Either Water or 1–2 ng of mRNA Encoding XNkx2-5 Full-length (FL) or Translation Termination Mutants Giving Rise to Carboxyl-End Truncated Proteins of 155 or 184 Amino Acids in Lengtha

Treatment n Mean atrial area Standard Deviation Standard error of the mean P value vs water
Water injected 27 9.2 2.77 0.533
Nkx2-5 FL 66 13.23 2.7 0.332 <0.001
Nkx2-5 His156ter 50 15.95 2.11 0.298 <0.001
Nkx2-5 Gln185ter 46 12.33 2.54 0.375 <0.001
a

All embryos were derived from the same pair of adult frogs. Embryos were fixed in Dent’s fixative and stained by whole-mount reactions with anti-troponin T monoclonal antibody followed by reaction with anti-mouse Alexa 568. Measurements were made by focusing on the widest part of the atrial field with a Zeiss Axiophot fluorescence microscope and capturing that image with a SPOT camera. Digital images were exported to NIH image, and the area of the atrial field was determined.