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. 2006 Nov 6;175(3):453–463. doi: 10.1083/jcb.200605144

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — SopE-mediated ruffling requires Rac1 but not Cdc42. (A–F) COS-2 cells were cotransfected with a plasmid expressing SopE, GFP (to detect transfected cells) along with RNAi constructs directed to Cdc42 (B) or Rac1 (C), or plasmids expressing Cdc42^N17 (D), Rac1^N17 (E), or vector control (A). 2 d after transfection, cells were stained with rhodamine-phalloidin, and the percentage of cells undergoing membrane ruffling as a consequence of SopE expression was enumerated by fluorescence microscopy (F). (F) Numbers indicate the percentage of transfected cells undergoing SopE-mediated actin remodeling (ruffling) and have been standardized considering the number of cells undergoing membrane ruffling in control transfections to be 100%. The results represent the mean ± SD (error bars) of three independent experiments. At least 200 transfected cells were counted for each independent transfection. Bar, 10 μM.