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. 2007 Feb 26;176(5):695–707. doi: 10.1083/jcb.200612129

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Copyright © 2007, The Rockefeller University Press

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Figure 3. — TGF-β1 regulates the expression of Notch ligands and receptors. (A) Kinetic expression profiles of JAG1 and NOTCH1 measured via microarray analysis (expressed in arbitrary units [au]). (B) Quantitative RT-PCR of JAG1,2, DLL1,2,3, and NOTCH1,2,3,4 mRNAs in HaCaT cells stimulated with 2 ng/ml TGF-β1 for 2, 6, 24, and 48 h in the absence (DMSO, −; gray lines) or presence of 4 μM GSI (+; black lines). Relative gene expression values (fold change) after normalization to GAPDH gene expression are shown. (C) Immunoblot analysis of JAG1, NOTCH1, NOTCH3, TβRI, and control β-tubulin protein levels from HaCaT cells treated with TGF-β1 and GSI as indicated. Immunoblots of total cell lysates (IB) or immunoblots after immunoprecipitation (IP/IB) are shown. Error bars represent SD.