Table III.
Primers used to tag various genes at their chromosomal loci
| Gene | Primer direction | Primer |
|---|---|---|
| PUF3 | Forward | 5′-TGTTGAGAAACTTGCAGCATTGGTTGAAAATGCGGAGGTGCGGATCCCCGGGTTAATTAA-3′ |
| PUF3 | Reverse | 5′-ATAGTAAAAAGTGAAAGGAGAACGATGATAACACTAATCAGAATTCGAGCTCGTTTAAAC-3′ |
| MDM12 | Forward | 5′-GCATGGCCAAGTTGGATTAATCTGGATTTCAACGATGCTGATGAGCGGATCCCCGGGTTAATTAA-3′ |
| MDM12 | Reverse | 5′-TTTATGTAGACACTATTTTCAAACTATCTTTGTTAAATTAGAATTCGAGCTCGTTTAAAC-3′ |
The sequences underlined in the primers correspond to the tagging plasmid sequence.