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Sequence analysis of the k43γ4 lethal mutant allele. The k43/dmOrc2p coding region was sequenced in the control (wild type) and k43γ4 mutant backgrounds, as indicated. The sequence of the region from nucleotide 115 to 133 is presented (5′-GGGAATGCAGTGGAATCTC-3′). The k43γ4 allele contains a substitution of CT for the G at position 121, as indicated by underlining. This mutation thus causes a frameshift of the k43γ4-encoded protein beginning at amino acid residue 41.
