TABLE 3.
Numbers and distributions of nucleotide and amino acid substitutions observed in the O Tw97 genome consensus sequence during passages in vivo and in vitroa
| Passage group | No. (%) of substitutions for indicated regionb
|
|||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lpro
|
P1
|
P2
|
P3
|
|||||||||||||||||||||
| VP4
|
VP3
|
VP2
|
VP1
|
2A
|
2B
|
2C
|
3A
|
3B
|
3C
|
3D
|
||||||||||||||
| nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | nt | aa | |
| In vivo | 6 (16) | 1 (11) | 0 | 0 | 1 (3) | 0 | 5 (13) | 1 (11) | 2 (5) | 0 | 0 | 0 | 2 (5) | 1 (11) | 10 (27) | 3 (33) | 1 (3) | 0 | 0 | 0 | 4 (11) | 1 (11) | 6 (16) | 2 (22) |
| In vitro | 3 (15) | 2 (12) | 1 (5) | 1 (6) | 1 (5) | 1 (6) | 1 (5) | 1 (6) | 4 (20) | 4 (25) | 0 | 0 | 1 (5) | 0 | 1 (5) | 1 (6) | 1 (5) | 1 (6) | 1 (5) | 1 (6) | 1 (5) | 1 (6) | 5 (24) | 3 (19) |
The total numbers of substitutions were 48 in vivo and 22 in vitro. Total numbers (percentages) of substitutions for subgroups are as follows: for UTRs in vivo, 11 (23); for ORFs in vivo, 37 (77); for UTRs in vitro, 2 (9); for ORFs in vitro, 20 (91); for nucleotides in P1 in vivo, 8 (22); for amino acids in P1 in vivo, 1 (11); for nucleotides in P2 in vivo, 12 (32); for amino acids in P2 in vivo, 4 (44); for nucleotides in P3 in vivo, 11 (30); for amino acids in P3 in vivo, 3 (33); for nucleotides in P1 in vitro, 7 (35); for amino acids in P1 in vitro, 7 (44); for nucleotides in P2 in vitro, 2 (20); for amino acids in P2 in vitro, 1 (6); for nucleotides in P3 in vitro, 8 (40); for amino acids in P3 in vitro, 6 (37).
nt, nucleotide; aa, amino acid.