FIG. 6.
(A) Sequence alignment of the nsp1 ZF-coding domain for a selection of EAV isolates (for details, see reference 64). The codons for the four key residues of the nsp1 ZF are boxed. (B) Structure of a predicted RNA hairpin that can be formed by the sequence specifying nsp1 ZF residues 22 to 28, thus including the highlighted codons for Cys-25 and His-27. Natural sequence variations (see panel A) are indicated in gray. Residues targeted for mutagenesis in the RNAko mutant (see text) are indicated with asterisks.