TABLE 1.
Group primers and capture probe information
| Serogroup | Gene target | Forward PCR primer | Reverse PCR primer | O-group-specific capture probe |
|---|---|---|---|---|
| Serotype Paratyphi A/D | prt | GA GTT TAT ATG CAT ATA CTA A | TAC TGG TAA ACT TAT C | GC GGC GGC GAA CTC ATT Ta/GC GGC GGC GAG TTC ATT Tb |
| TGC TGG TAA ATT TAT Cc | GT GGC TGC GAG TTC ATT Tc | |||
| B | abe | GC ATA AAA CAT CAT CTC TCA TAA A | CGC ATA TGT TGA TAA TTA AAA TCT TT | TT TAT TGC CAA ATC AAG AAG C TT TAA TGG TTT TA |
| C1 | wzy | GC TTT AAT CCA ATA TAA AA | TAA ATA TAT GAT AGT TCC AAA TAA | AT GAA ATG TCG ACG CAC ATA GAA TAA GAA TAA GC |
| C2 | abe | CA GAC GTT TGT TTT ATA A | GAT CGT ACA ATC AAT ATC | TC CCT CTC ACC GTA AAC ATG TTC TAA ACG TAA ATT |
| E | wzx | CT GAT TTA ACC GGG TAT TAT TTA T | CTT TTC TAT GCA GTG GTT TAA | AG GAT ATA TGT TAA GGC TGT ACT TAT ACC TAG CT |
| O13 | wzy | CT CTT GAT GAA TGT TAT TA | GTT AAC CCC TCC TAA TA | AT ATA TAG ATG TTT ATT ATA TTT GCA TTC CCC A |
a
Serogroup A probe.
b
Serogroup D probe.
c
Reverse primer and probe sequence for subspecies II serogroup D.