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. Author manuscript; available in PMC: 2008 Oct 4.
Published in final edited form as: Neuron. 2007 Oct 4;56(1):66–78. doi: 10.1016/j.neuron.2007.08.008

Figure 7. AICD Binds To and Suppresses LRP1 Promoter Activation.

Figure 7

(A) Schematic diagram of LRP1 promoter-luciferase construct. (B) BHK570 cells were transiently co-transfected with the LRP1 promoter-Luc construct together with control vector, AICD, Fe65, AICD/Fe65, AICD mutant (Y682G), AICD mutant/Fe65, or NICD. LRP1 promoter-driven luciferase activity was significantly decreased by AICD and Fe65 and further by the co-expression of both, but not by an AICD mutant or NICD. (C) APP/APLP2-DKO MEF cells were transiently co-transfected with the LRP1 promoter-Luc construct together with control vector, AICD, Fe65, or both, and the luciferase activity was measured as in (B). (D) ChIP assay showed that antibodies to AICD, Fe65 and Tip60, but not control IgG, immunoprecipitate LRP1 promoter DNA fragment. Notch target HES1 promoter was used as a negative control. The ability of anti-Fe65 and anti-Tip60 to immunoprecipitate LRP1 promoter DNA is greatly reduced in APP-KO mouse brain. (E) Quantitative real-time PCR analysis of LRP1 promoter DNA immunoprecipitated by control IgG or antibodies to AICD, Fe65 and Tip60.