FIGURE 3.
Plus end retrograde RNA motility is enhanced in Glued mutant embryos. In Glued1 mutant embryos, ftz RNA particles display shorter minus runs, longer plus runs, and travel more frequently toward the plus ends compared with wild type. (A) Graph of minus and plus runs in wild type. (B) Graph of minus and plus runs distribution in Glued1. The runs of each RNA particle are grouped and displayed in a distinct color along the X-axis, and pauses are represented as gaps between the runs. The Y values represent microns of distance covered in both directions, along the Y-axis. To achieve this, the images were first rotated so that the Y axis was parallel to the apico-basal axis of the embryos. Runs toward the minus ends of MTs (forward runs) are shown above the X axis and runs toward the plus ends of MTs (backward runs) are shown below the X axis. (C) Proportion of distance covered toward the minus and plus directions in wild type and Glued1 embryos. (D) Frequency of plus runs in wild type and Glued1 embryos. (E) Average length of minus and plus runs in wild type (4.48 ± 0.023 μm and 0.592 ± 0.0034 μm, respectively) and Glued1 embryos (2.401 ± 0.014 μm, P = 0 and 0.86 ± 0.003 μm, P = 0.033) (F) Mean speed of minus and plus runs in wild type (0.74 and 0.54 ± 0.01 μm/sec, respectively) and Glued1 embryos (0.61 μm/sec, P = 0.048 and 0.36 μm/sec, P = 0.067, respectively). Values shown are mean values for all runs analyzed in a given genetic background ± SEM. P shows t-test probability compared to wild type. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001.