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. Author manuscript; available in PMC: 2008 Aug 31.
Published in final edited form as: J Immunol Methods. 2007 Jun 12;325(1-2):20–34. doi: 10.1016/j.jim.2007.05.011

Figure 3. Optimal conditions for detecting degranulating CD8+ T lymphocytes in rhesus macaques.

Figure 3

PBMC were stimulated with SEB and stained with antibodies to (A) CD107a (PE), (B) CD107b (FITC), or (C) both CD107a and CD107b, present throughout the stimulation period (“during”), or added at the end of the stimulation period (“post”). Gated CD8+ T lymphocytes are shown. (D) Comparison of surface LAMP expression when antibodies were added “during” or “post”. Data following 6 or 18 hours of SEB and rhCMV-CD8 peptide stimulation are shown. P-Values were calculated by the nonparametric Wilcoxon Signed Rank test.