Figure 2. Cytokine secretion by resident peritoneal macrophages upon WGP or zymosan stimulation.
(A) C57Bl/6 WT resident peritoneal macrophages (5×105) were stimulated for 3 hrs in the presence or absence of WGPs (100 μg/ml) before adding BD GolgiPLUG. The cells were cultured for an additional 3 hrs, then harvested and stained with mAb F4/80-PE-Cy5.5. After washing, cells were fixed, permeabilized, and stained with the indicated anti-cytokine mAbs or isotype control. (B) Resident macrophages (5×105) were stimulated with WGPs, F1-5, F5-14, or PGG β-glucan (100 μg/ml) or zymosan (100 μg/ml) for 3 hrs. The supernatants were collected and detected for cytokines using BD Cytometric Bead Array (CBA) kit. Mean ± SE of duplicates are shown. Data represent one representative of two independent experiments.