Figure 4.
Specificity of HLA-H protein immunostaining demonstrated in pyloric antrum (a–c) and duodenum (d–f). Sections a and d were stained using anti-HLA-H protein antibody. The positive immunoreaction is seen in the surface epithelium of pyloric antrum, the cryptal epithelium of duodenum, and subepithelial leukocytes (small arrowheads). The positive reaction was blocked in both tissues by addition of the C-terminal peptide (50 μg/microscope slide) (b and e). Sections c and f show control staining with 1:100 diluted normal rabbit serum. Small arrowheads, leukocytes; large arrowheads, Brunner’s glands. (×200.)