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HCT116 cells were transfected with control or eEF2k siRNA. Cells were serum starved and treated for 3 h with 10 μM LPA, 100 nM PMA, or 15% FBS. Cells were lysed and the expression levels of KLF5, eEF2k, and β-actin were determined by Western blot. Representatives of three separate experiments are shown.
